Figure 3.

(A) Schematic representation of His₆-eIF4B fragments. Six contiguous hexahistidine-tagged fragments comprised of ∼100 residues each were generated. The RRM, DRYG domain and ARM RNA-binding domain are indicated. (B) Recombinant fragments were purified by Ni²⁺ affinity chromatography, and then subjected to SDS–PAGE and Coomassie blue staining. (C) Equal amounts of the same fragments were phosphorylated by S6K1 in vitro. Lane numbers indicate eIF4B fragment number. The arrow indicates autophosphorylated S6K1.