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. 2004 Apr 8;23(8):1761–1769. doi: 10.1038/sj.emboj.7600193

Figure 7.

Figure 7

Ser422 phosphorylation (or phosphorylation mimic) is required to inhibit translation. 293T cells were cotransfected with a reporter construct encoding human GH and various Flag eIF4B constructs. (A; top) Northern blot of total RNA probed with a human GH DNA segment. (Middle panel) Western analysis using the anti-Flag antibody. (Bottom panel) Western analysis using an anti-hGH antiserum. (B) Polysome profile analysis of human GH mRNA from cells transiently transfected with pS16-GH and Flag-eIF4B S422A (top panel) or eIF4B S422E (bottom panel). Locations in the gradient of 40S, 60S and 80S ribosomes, and polysomes are indicated.