Figure 3. NF2 isoforms 1 and 2 display distinct localizations within the apical domain.

Wing imaginal discs expressing Flag-tagged NF2^Iso1 (A–C) or NF2^Iso2 (D–F) under the ApGal4 driver. Images are taken along the dorsal-ventral boundary to include the expression boundary of ApGal4, thereby demonstrating the specificity of anti-Flag staining in these tissues. Comparison to aPKC, a marker for the marginal zone in these cells (A,D), demonstrates that NF2^Iso1 (B–C) is expressed in the apical cell cortex but is not closely associated with the marginal zone. In contrast, NF2^Iso2 (E–F) is highly concentrated in the marginal zone together with aPKC.