Figure 1.

Co-stimulation of DC with LPS and curdlan increased IL-23 production. (A and B) BMDC prepared from C57BL/6 mice were stimulated with LPS (1 µg/ml), curdlan (100 µg/ml), or both. IL-23 was measured by ELISA at 24 h (left panels). mRNA levels of IL-23p19 (middle panels) and IL-12p40 (right panels) were determined at the indicated time by qRT-PCR. (C) IL-10 was measured by ELISA (left panel). IL-23 was measured by ELISA (middle panel). Cells were cotreated with LPS and curdlan in the presence of α-IL-10R (15 µg/ml) or rat IgG1 (15 µg/ml). BMDC prepared from C57BL/6 (Il10^+/+) or Il10^+/+ mice were treated with LPS, curdlan, or both and IL-23 was measured by ELISA (right panel).