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. 2014 Feb 21;14(1):30–37. doi: 10.4110/in.2014.14.1.30

Figure 3.

Figure 3

Co-treatment of DC with LPS and curdlan was unable to enhance Th17 differentiation. CD4+ T cells from C57BL/6 mice were stimulated with plate-bound anti-CD3 and anti-CD28 in the presence of conditioned media (CM) as described in the Materials and Methods. CM were prepared by treating BMDC with indicated stimulants for 24 h. T cell cultures were then restimulated on day 5 with plate-bound anti-CD3 for 48 h. ELISA was performed to detect IL-17A, IFN-γ and IL-4 production. *UT: CM from untreated DC.