Skip to main content
. 2014 Feb 21;14(1):30–37. doi: 10.4110/in.2014.14.1.30

Figure 4.

Figure 4

Exogenous TGF-β but not IL-6 nor IL-1β enhanced IL-17 production in co-stimulated DC. (A) BMDC were stimulated with LPS (1 µg/ml), curdlan (100 µg/ml), or both. Total TGF-β1 and bioactive TGF-β were determined as described in the Materials and Methods. *Media: DC culture media (RPMI+5% serum); UT: CM from untreated DC. (B) CD4+ T cells were stimulated in the presence of CM plus indicated cytokines (IL-6 (40 ng/ml), IL-1β (+: 1 ng/ml, ++: 10 ng/ml), TGF-β (+: 2.5 ng/ml, ++: 5 ng/ml, +++: 25 ng/ml)). IL-17A was measured by ELISA after anti-CD3 restimulation. (C) CD4+ T cells were stimulated in the presence of CM with or without TGF-β (0.5 ng/ml). Cultures were restimulated for 6 h on day 5 with plate-bound anti-CD3 and mRNA levels were assessed by qRT-PCR.