Skip to main content
. Author manuscript; available in PMC: 2014 Mar 5.
Published in final edited form as: J Med Chem. 2013 Apr 12;56(8):3419–3423. doi: 10.1021/jm301456c

Figure 2.

Figure 2

Antinociceptive results of hot plate and tail-flick in vivo bioassays for compound 1, Biphalin, and morphine sulfate. Compounds were injected by icv administration (A,B) at a dose of 0.6 nmol, and systemic iv administration (C,D) at a dose of 3000 nmol. The data represent the mean ± SEM. The statistical significance among groups was determined, in comparison with vehicle-treated animals with the analysis of variance (two-way ANOVA test) followed by Bonferroni’s posthoc comparisons using the statistical software SPSS. Statistical significance was P < 0.05 (*P < 0.05; ***P < 0.001).