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. 2014 Mar 5;34(10):3475–3492. doi: 10.1523/JNEUROSCI.4768-13.2014

Figure 3.

Figure 3.

VGLUT1-IR contacts on MN dendrites display the active zone marker Bassoon. A, Single confocal optical section through the cell body and proximal dendrite of a neurobiotin filled MN (Cell #7). The section was counterstained for VGLUT1 (FITC, green) and bassoon (DyLight 649, blue). B, C, High-magnification of the VGLUT1-IR contact; box in A. Bassoon immunoreactivity is detected in between the VGLUT1-IR contact and the MN dendrite. A line density profile was taken at the indicated position. D, Line profile density. A bassoon immunoreactivity peak (blue line) is present between the peak of VGLUT1-immunofluorescence (green line) and the neurobiotin-filled dendrite (red line). Scale bar (in A), 20 μm.