Figure 3.

Amyloidogenic processing in non-neuronal cells. A, Western blot analyses of ACM, MCM, or NCM cultures (150 μl; 3% of total volume) from mentioned transgenic genotypes, immunoblotted with 26D6 antibody. B, Western blot analyses of cell lysates (top 2 rows) or CM (third row) prepared from astrocyte, microglia, or NPC cultures established from APPswe/PS1ΔE9flox genotype after treatment with L-685,458 (1 μm) were immunoblotted with 369 antibody to detect full-length APP (FL APP) and C-terminal fragments (APP CTF) or 26D6 antibody. C, Western blot analyses of Aβ₄₀ and Aβ₄₂ peptides, immunoprecipitated with 6E10 from CM of APPswe or APPswe/PS1ΔE9flox microglia, were immunoblotted against 26D6. A total of 0.2 ng of pure Aβ₄₀ and Aβ₄₂ peptides were loaded as controls (lanes 1, 2).