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. 2014 Mar 5;34(10):3674–3686. doi: 10.1523/JNEUROSCI.3703-13.2014

Figure 1.

Figure 1.

HuD-GFP is expressed in lower- but not upper-layer primary neurons of the mature neocortex. A–E, Representative 10× confocal images of the neocortical wall for DAPI (blue), HuD-GFP (green), Tle4 (red), Cdp (light blue), and merged channels, respectively. Dashed line demarcates upper versus lower neocortical layers. UL, Upper layers; LL, lower layers. For A′–E′, insets are representative 60× confocal images of upper neocortical layers; for A′′–E′′, insets are representative 60× confocal images of lower neocortical layers. White arrows indicate HuD-GFP/Tle4+ neurons. Yellow arrows indicate HuD-GFP+/Tle4− neurons. F, F′, Representative light microscopy image of HuD-GFP mouse sagittal brain section using anti-GFP-DAB staining (Gong et al., 2003). G, Quantification of the proportion of HuD-GFP+ neurons colocalized with Tle4 (left) and Tle4+ neurons colocalized with HuD-GFP (right).