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. 2014 Mar 5;34(10):3674–3686. doi: 10.1523/JNEUROSCI.3703-13.2014

Figure 6.

Figure 6.

HuD controls the earliest stages of dendrite outgrowth. A–B′, Schematic of In utero electroporation and dissociation of Ctrl shRNA/RFP (top) and HuD shRNA/GFP in E13.5 developing neocortex. Developing neocortices were electroporated at E13.5 with either Ctrl shRNA (RFP) or HuD shRNA (GFP). After 4 h, neocortices were dissociated and cultured. C, Schematic of dissociation of electroporated neocortices for primary cell culture. D, D′, Schematic of cell cultures taken at 1 and 3 DIV for analysis. E, F, Representative 60× confocal images of Ctrl and HuD shRNA transfected neurons at 1 DIV, respectively. G, H, Representative 60× confocal images of Ctrl and HuD shRNA transfected neurons at 3 DIV, respectively. I–L, Quantification of neurite endings in 1 and 3 DIV cell cultures. M, qRT-PCR analysis of HuD shRNA efficiency in vitro. Gapdh was used as a normalization control.