Figure 5.

CIE does not alter D2/D4 receptor expression in the mPFC. A, Coronal view of the rat brain atlas of the mPFC showing the region (gray box) of analysis of D2/D4 receptor expression. B, Quantitative autoradiography of D2 receptor binding using [³H]raclopride (left graph) and D4 receptor binding with [³H]nemonapride (right graph) demonstrating no difference in specific binding between control and CIE-exposed rats (1 week withdrawal; n = 8/group; p = 0.60, unpaired t test). C, CIE exposure was associated with an increase in D2 receptor-stimulated [³⁵S]GTPγS binding (CIE: n = 7; control: n = 6; *p < 0.02, unpaired t test). D, CIE exposure did not alter the number of D2 receptor-IR-positive cells in the mPFC (n = 6/group; p = 0.27, unpaired t test). Scale bar, 100 μm.