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. 2014 Mar 5;34(10):3719–3732. doi: 10.1523/JNEUROSCI.4206-13.2014

Figure 2.

Figure 2.

Kv7.2 and Kv7.3 colocalize at AISs and nodes of Ranvier of functionally identified L5 pyramidal neurons. A, Top, z-projected confocal images of the soma and AIS morphology (red, Alexa Fluor 488 fill) and immunofluorescence staining of Kv7.2 (cyan) and Kv7.3 (green) in RS and IB neurons. White arrows indicate the region of the Kv7 immunostaining. Images are background subtracted and pseudo-colored. Scale bars, 10 μm. Bottom, Nodes of Ranvier (NoR) indicated by axonal bifurcation points of identified RS and IB neurons. White arrows indicate positive staining for Kv7.2/7.3 at the NoR. Scale bars, 3 μm. B, Example of a regular firing neuron that was converted into intrinsic burst firing during bath application of XE-991 (red). Calibration: 0.1 s, 20 mV. C, Scatter and bar plots of RS and IB groups show a similar increase in high-frequency action potentials after application of 20 μm XE-991. Scatter plot, Individual experiments. Bars represent mean ± SEM.