Table 1.

Calculated number of adherent cells (n = 3)

Time of recovery (h)	0.5 h		2 h		24 h
	Mean number living cells	Total cells evaluated	Mean number living cells	Total cells evaluated	Mean number living cells	Total cells evaluated
Time of cultivation (h)a
Control	20,253 ± 377*	21,225 ± 385	10,809 ± 200**	11,630 ± 205	21,227 ± 327*	22,146 ± 332
0 h	13,577 ± 271*	16,385 ± 294	11,608 ± 155**	14,763 ± 164	10,089 ± 187*	13,767 ± 225
24 h	8,738 ± 142	11,805 ± 170	9,121 ± 178	12,023 ± 210	10,340 ± 170	15,693 ± 244
48 h	10,896 ± 185	13,328 ± 199	13,050 ± 162	15,485 ± 179	15,728 ± 261	20,123 ± 232
Mean number of living cells, normalized to control (%)b
0 h	63.97 ± 1.99		99.81 ± 1.34		45.56 ± 1.85
24 h	41.17 ± 1.63		78.43 ± 1.95		46.69 ± 1.64
48 h	51.34 ± 1.69		112.21 ± 1.24		71.02 ± 1.66

^aMean number of living and total evaluated cells

^bNormalized number of living cells to control (%)

* Cell number recovered after 0 h were statistically significant less than cell number adherent on non-frozen control cryogels after 0.5 h and 24 h cultivation (P < 0.05)

** Difference in cell number before and after cryopreservation of 2 h cultivation cryogels was statistically not significant (P < 0.05)