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. Author manuscript; available in PMC: 2014 Aug 28.
Published in final edited form as: Nat Commun. 2014 Feb 28;5:3350. doi: 10.1038/ncomms4350

Figure 3. Mutagenesis reveals the predominant orientation for the FLIP-FADD interaction.

Figure 3

(a) Western blot analysis of GST pull-down assay using FADD DED as bait and wild-type and mutant human Flag-FLIP(S) as prey. (b) Western blot analysis of GST pull-down assay using FADD DED as bait and wild-type and mutant human Flag-FLIP(L) as prey. (c) Western blot analysis of GST pull-down assay using FADD DED as bait and His-tagged recombinant wild-type and F114Y mutant FLIP(S) as prey. (d) Western blot analysis of GST pull-down assay using FLIP(S) as bait and wild-type and mutant Flag-FADD as prey. (e) Western blot analysis of His pull-down assay using His-FLIP(S) as bait and wild-type and H9A and H9G mutant Flag-FADD as prey.