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. 2014 Mar 5;5:43. doi: 10.3389/fgene.2014.00043

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Copyright © 2014 Amaral, Brito, Chobanyan, Yoshikawa, Yokokura, Van Vactor and Gama-Carvalho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcriptome profiling of Smn^wt/elavGAL4 (WT) and Smn^wt/elavGAL4-C24 (C24) Drosophila strains. (A) Correlation between samples estimated from normalized counts of gene expression of ~12,000 genes in WT (N = 3) and C24 (N = 4) paired-end mRNA-seq libraries. Font size is varying according to the value of correlation. (B) Overview of hierarchical clustering of mRNA-seq datasets. Hierarchically clustered gene expression profiles were performed using the estimation of stabilized variance of normalized counts of gene expression as in (A). Genes are ordered on the vertical axis. Values are displayed as a Z-score (mean = 0 and SD = 1) across samples. The magnitude of deviation from the median is represented by the color saturation. We observed that samples do not cluster according to the known biological origin.