FIG. 1.

Schematic diagram of the Fus1 protein domain structure deduced from multiple alignment shows key elements of the myristoyl-switch protein family. (A) The amino-terminal sequence of Fus1 fits consensus for EF-hand domains in recoverin and mitochondrial Ca²⁺-binding proteins. (B) Alignment of the Fus1 N-terminal fragment (pos. 45-110 a.a) and a myristoyl-binding domain of recoverin. Conserved positions are shown in shaded rectangles; chemically similar amino acids are indicated with open rectangles. Key amino acids that compose the myristoyl-binding hydrophobic pocket of recoverin are marked with asterisks and presented in red if they are identical or chemically similar to the corresponding residues in the Fus1 protein. (C) Schematic representation of the Fus1 protein domain structure. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars