FIG. 3.

Fus1 is required for the maintenance of basal mitochondrial homeostasis and for mitochondrial response to Ca²⁺ overload in immune cells. (A) Fus1-dependent changes in basal mitochondrial parameters coupled with Ca²⁺ dynamics measured in splenocytes isolated from Fus1 WT and Fus1 KO mice (n=5). Results are expressed as fold changes relative to WT cells. (B) Dynamics of changes in major mitochondrial parameters upon increasing of calcium load in splenocytes of WT and Fus1 KO mice (n=5–6/group). Results are presented as fold changes in relative fluorescence (RF) values compared to control cells normalized to 1.0. (C) Analysis of the efficiency of transduction of coupled mitochondrial parameters in WT and KO splenocytes under conditions of increasing calcium load. Correlation values were calculated as IC50 and presented as mean±SE. IC50 was calculated in accordance with general rules applied for determination in values of effectiveness of parameters transformation restored from a series of dose–response data as described elsewhere (45). Data represent mean±SE; p-values are designated as *, <0.05; **, <0.01; ***, <0.001. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars