FIG. 5.

Fus1 modulates Ca²⁺ mobilization-dependent processes during CD4⁺ T- cell activation. (A, B) Effect of Fus1 loss on the dynamic changes in surface levels of CD4 and (B) PD-1/PD-L1 molecules during CD3/CD28 stimulation of WT and Fus1 KO CD4⁺ T lymphocytes. (C–E) Fus1-dependent changes in the effector functions of activated CD4⁺ T cells, such as Th differentiation, which was defined based on preferential expression of IFNγ (Th1 cell marker) or IL-4 (Th2 cell marker) (C), proliferation (D), and apoptosis (E). Data represent mean±SE; p-values are designated as *, <0.05; **, <0.01; ***, <0.001. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars