FIG. 3.

Mutation in FUS gene abrogates MnSOD transcription. Luciferase activity of the MnSOD promoter (−3401 to +24)-driven pGl3 reporter gene after transfection with wild-type or mutant FUS expression vector (A). Relative level of MnSOD mRNA was determined by real-time PCR after normalization with GAPDH mRNA levels as loading control (B). MnSOD enzyme activity (Units/mg protein) was measured in whole cell extract after transfection of wild-type FUS or mutant FUS expression vector in JB6 cells (C). Each data point represents mean±SD of three independent experiments and significant difference as compared to respective controls is indicated by *p<0.05 and **p<0.01.