Figure 4.

Growth effects of H₂O₂ elimination on PC-3 and PNT1A cells. Cell numbers were measured using crystal violet following a 72 h continuous exposure, and are displayed as % cell growth compared to untreated cells. The displayed data represent the average ±SEM of three independent experiments. Dose-dependent growth inhibition by catalase was observed in both cell lines, with PC-3 cells requiring higher concentration of catalase (IC₅₀ = 580 ± 160 U/mL) to block cell proliferation, and PNT1A cells requiring lower concentration of catalase (IC₅₀ = 75 ± 40 U/mL). The IC₅₀ values were calculated using curve fit (GraphPad Prism) and applying a non-linear fit log (C_inhibitor) versus normalized response.