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Non-IgE mediated mast cell IL-33 expression. Toll-like receptor-2 (TLR-2) (100 μg/mL zymosan; Sigma), TLR-9 [1 μM CpG-Oligonucleotide (ODN1668); InvivoGen] mediated IL-33 kinetic mRNA expression using real-time RT-PCR over a time course of 24 h. Data are representative of 3 independent experiments.
