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. 2014 Mar 1;34(3):141–147. doi: 10.1089/jir.2012.0066

FIG. 4.

FIG. 4.

IL-33-induced mast cell signaling events. (a) Western blotting analysis of mast cells ERK, p38, JNK, and nuclear factor-kappa B (NFκB)-p65 activation after 10 ng/mL rmIL-33 treatment over a time course of 30 min. To further test the downstream signaling-induced functional effects, specific inhibitors for JNK (SP600125), ERK (PD98059), p38 (SB203580), and NFκB (Bay11–7082) at different dosages were added to BMMCs 30 min before rmIL-33 treatment. Supernatants were collected to test for the inhibitors' effects on IL-33-induced mast cell (b–e) IL-6 and (f–i) IL-13 production.