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. 2014 Jan 27;6(2):489–503. doi: 10.3390/nu6020489

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Effect of RVT on Δψ_m. Spermatozoa were obtained from ductus deferens in M-16 medium, pooled and incubated with RVT, Fe²⁺/asc or RVT + Fe²⁺/asc. Δψ_m was determined using the Mito ProbeJC-1 assay kit and the fluorescence was measured on a spectrofluorometer. Values represent the mean ± SD of three independent experiments performed in duplicate. * p < 0.05 vs. control group. ** p < 0.05 vs. Fe²⁺/asc group.