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Effect of RVT on SOD activity. Spermatozoa were obtained from ductus deferens in M-16 medium, pooled and incubated with RVT, Fe2+/asc or RVT + Fe2+/asc. Enzymatic activity of SOD was measured with a spectrophotometer after using the RANSOD assay kit. Values represent the mean ± SD of three independent experiments performed in duplicate. No significant differences were observed.
