Table 2. Effects of tag insertions on IBa parameters during 3-s depolarising pulses.
| τfast [ms] | τslow [ms] | %τfast | n | 350 ms [%] | 1000 ms [%] | 2000 ms [%] | n | |
|---|---|---|---|---|---|---|---|---|
| wild-type CaV2.1 | 166.3 ± 25.4 | 758.3 ± 78.7 | 84.2 ± 4.6 | 6 | 78.6 ± 3.2 | 94.6 ± 1.6 | 97.3 ± 1.9 | 11 |
| 1722-HA | 168.2 ± 29.1 | 749.7 ± 89.3 | 83.4 ± 3.6 | 10 | 79.4 ± 4.6 | 95.0 ± 1.2 | 97.8 ± 1.2 | 12 |
| wild-type CaV2.1 | 172.7 ± 21.8 | 801.0 ± 152.2 | 86.2 ± 4.6 | 8 | 79.8 ± 4.1 | 95.1 ± 1.5 | 97.6 ± 1.4 | 15 |
| 1722-HAGHA | 161.3 ± 20.8 | 880.1 ± 166.5 | 88.1 ± 4.9 | 8 | 80.1 ± 6.4 | 95.6 ± 1.6 | 97.9 ± 1.0 | 15 |
| wild-type CaV2.1 | 183.1 ± 33.4 | 875.6 ± 219.7 | 84.4 ± 4.7 | 30 | 77.8 ± 4.5 | 94.3 ± 1.7 | 97.3 ± 1.7 | 58 |
| 1722-9His | 184.7 ± 45.1 | 863.6 ± 144.7 | 82.2 ± 6.6 | 43 | 80.7 ± 4.8b | 95.9 ± 2.5a | 99.0 ± 2.1a | 41 |
| wild-type CaV2.1 | 173.0 ± 17.6 | 793.9 ± 148.1 | 85.9 ± 5.5 | 5 | 79.7 ± 4.1 | 95.1 ± 2.2 | 97.5 ± 1.9 | 12 |
| 1727-9His | 124.4 ± 20.2a | 743.1 ± 136.1 | 88.1 ± 2.5 | 11 | 87.2 ± 2.7a | 97.0 ± 1.0c | 99.0 ± 1.3c | 13 |
| 1732-9His | 126.1 ± 12.6a | 772.7 ± 93.8 | 87.1 ± 1.9 | 11 | 86.3 ± 1.6a | 97.0 ± 1.5c | 99.4 ± 1.7c | 12 |
| 1732-HA | 157.8 ± 18.3 | 815.9 ± 141.5 | 88.5 ± 2.4 | 6 | 80.0 ± 4.4 | 95.3 ± 1.7 | 97.7 ± 1.6 | 13 |
| wild-type CaV2.1 | 166.3 ± 25.4 | 758.3 ± 78.7 | 84.2 ± 4.6 | 6 | 78.6 ± 3.2 | 94.6 ± 1.6 | 97.3 ± 1.9 | 11 |
| 280-bbs | 147.9 ± 15.9 | 857.4 ± 147.1 | 86.3 ± 2.6 | 9 | 82.4 ± 2.0b | 95.6 ± 1.3 | 98.2 ± 1.1 | 9 |
| 1722-bbs | 200.4 ± 49.7 | 867.8 ± 117.9 | 82.4 ± 6.7 | 5 | 67.8 ± 9.3b | 92.3 ± 2.8c | 96.9 ± 1.7 | 11 |
Inactivation after a 3-s depolarising pulse to Vmax was fit to a bi-exponential decay yielding time constants for both the fast (τfast) and the slow (τslow) component, as well as the contribution of the fast component (%τfast). Because of the high variability of measurements calculations did not reveal significance though obvious from graphical analysis, we calculated % inactivation after 3 predefined time points (350 ms, 1 s, 2 s) from the same data sets and analysed it statistically. Data are means ± SD for the indicated number of experiments. Statistical data were obtained by Student's t test and are indicated as
p < 0.001
p < 0.01
p < 0.05.
Wild-type data from experiments carried out with the same batches of injected oocytes are given as controls for each construct.