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. Author manuscript; available in PMC: 2014 Mar 5.
Published in final edited form as: J Am Coll Cardiol. 2013 Mar 5;61(9):971–980. doi: 10.1016/j.jacc.2012.12.018

Figure 5. S6K Controls Proliferation by Regulating Cyclin D1 Expression.

Figure 5

(A) HAECs were incubated with SRL (500 nmol/l) or Mf (30 mmol/l), alone and in combination, and Western blots performed for cyclin D1. Relative expression normalized to beta-actin levels were measured by densitometry (n = 4 per group). (B) HAECs were transfected with siRNA for S6K1 and HAECs were transfected with siRNA for S6K1 and nontargeting, scrambled siRNA (Scr). Cyclin D1 mRNA expression measured by quantitative polymerase chain reaction (n = 4 per group). Cyclin D1 mRNA expression measured by quantitative polymerase chain reaction nontargeting, scrambled siRNA (Scr) (n = 4 per group). (C, D) Cyclin D1 was overexpressed in HAECs via plasmid transfection and exposed to increasing doses of Mf (0 to 30 mmol/l) or SRL (0 to 10 nmol/l), respectively, with cell proliferation measured at 24 h (n = 4 per group). Abbreviations as in Figures 1 through 3.