(A) HAECs were incubated with SRL (500 nmol/l) or Mf (30 mmol/l),
alone and in combination, and Western blots performed for cyclin D1. Relative
expression normalized to beta-actin levels were measured by densitometry (n = 4
per group). (B) HAECs were transfected with siRNA for S6K1 and
HAECs were transfected with siRNA for S6K1 and nontargeting, scrambled siRNA
(Scr). Cyclin D1 mRNA expression measured by quantitative polymerase chain
reaction (n = 4 per group). Cyclin D1 mRNA expression measured by quantitative
polymerase chain reaction nontargeting, scrambled siRNA (Scr) (n = 4 per group).
(C, D) Cyclin D1 was overexpressed in HAECs via plasmid
transfection and exposed to increasing doses of Mf (0 to 30 mmol/l) or SRL (0 to
10 nmol/l), respectively, with cell proliferation measured at 24 h (n = 4 per
group). Abbreviations as in Figures 1
through 3.