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. Author manuscript; available in PMC: 2014 Mar 5.
Published in final edited form as: J Immunol. 2009 Apr 1;182(7):3985–3994. doi: 10.4049/jimmunol.0803053

Fig. 3. CD4+ T cells that upregulate CD25 expression during acute T. gondii infection are Th1 effector cells that produce high levels of IFN-γ.

Fig. 3

CD4+CD25− and CD4+CD25hi splenic T cells were sort purified from (A, B) naïve mice or (B–F) T. gondii infected mice (day 10 of infection). CD25 detection was performed using the PC61 clone anti-CD25 antibody. CD4+CD25− and CD4+CD25hi cells were cultured with (A–D) naïve APC and anti-CD3 antibody or (E–F) T. gondii pulsed APC. (A, C, E) IFN-γ production was assayed in supernatant by ELISA. (B, D, F) Proliferation was determined by 3H Thymidine incorporation. The results shown are the mean ± std. dev. of the group (n = 3) and are representative of 2 independent experiments. # denotes significant difference between CD4+CD25− and CD4+CD25hi cultures (p<0.05). * denotes significant difference between CD4+CD25− and CD4+CD25− and CD4+CD25hi co-cultures (P<0.05).