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. Author manuscript; available in PMC: 2014 Mar 5.
Published in final edited form as: Head Neck. 2008 Jun;30(6):790–799. doi: 10.1002/hed.20770

FIGURE 6.

FIGURE 6

Evaluation of thymidine kinase-1 activity. (A) Western blotting for TK1 expression with actin loading control. (B). Western blotting with the indicated antibodies following A431 and SCC1 cell siRNA oligonucleotide transfection for TK1 or firefly luciferase (Luc). (C) Parallel 3H-FLT evaluation of TK1 knockdown on FLT accumulation (mean ± SEM of 3 independent experiments). (D) The effects of erlotinib treatment of A431 cells on relative TK1 protein levels by Western blot, and parallel 3H-FLT uptake. Mean TK1 protein levels, normalized to β-actin, and corresponding levels of 3H-FLT uptake are shown as mean ± SEM of 3 independent experiments.