Fig. 4.

Effects of arachidonic acid, γ-linolenic acid or linoleic acid on glucose-induced insulin release. In pancreatic perfusion experiments, an equilibration period of 20 min preceded time 0. After a baseline period of 10 min, 10 mM glucose with arachidonic acid, γ-linolenic acid or linoleic acid (100 µM) was administered for 30 min followed by basal medium (KRB) perfusion. The horizontal line indicates the presence of arachidonic acid, γ-linolenic acid, linoleic acid and glucose, respectively. Values are means ± SE (n=4). Baseline effluent concentrations of insulin were 4,172 ± 277 pg/ml, 2,881 ± 775 pg/ml, 1,850 ± 189 pg/ml, 2,546 ± 198 pg/ml and 1,626 ± 298 pg/ml for the control, 10 mM glucose, 100 µM arachidonic acid with 10 mM glucose, 100 µM γ-linolenic acid with 10 mM glucose and 100 µM linoleic acid with 10 mM glucose groups, respectively.