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. 2014 Mar 15;141(6):1272–1281. doi: 10.1242/dev.105908

Fig. 1.

Fig. 1.

miR-142-3p regulates mesenchymal cells proliferation and differentiation. (A) MicroRNA microarray shows miRNAs differentially expressed in E12.5 lung epithelium (blue) versus mesenchyme (red). miR-142-3p (arrowhead) is specifically upregulated in the embryonic lung mesenchyme. (B) Expression of miR-142-3p in the epithelium and mesenchyme of E12.5 lungs analyzed by qPCR. (C-J) miR-142-3p LOF assay on E11.5 lung explants treated with scramble vivo-morpholino (scra; C-F) and miR-142-3p vivo-morpholino (mo142; G-J). (K) Expression of miR-142-3p detected by qPCR 48 hours after treatment with mo142. (L-N) Morphometric analysis 48 hours after miR-142-3p LOF assay on E11.5 lung explants. (O-R) Immunostaining for KI67 showing cell proliferation in scra- (O,P) and mo142 (Q,R)-treated E11.5 lung explants, and quantification of proliferation in the epithelium and mesenchyme (S). (T-W) Immunostaining for ACTA2 showing smooth muscle cells at the tips of scra- (T,U) and mo142 (V,W)-treated E11.5 lung explants. Dashed boxes in O, Q, T and V indicate the magnified areas in P, R, U and W, respectively. White arrowheads indicate ectopic ACTA2 expression. (X) Quantification of ACTA2 expression at the tip of the lung explants from the previous experiment. Scale bars: 250 μm (C,E,G,I); 50 μm (D,F,H,J); 100 μm (O,Q,T,V); 25 μm (P,R,U,W). Data are means ± s.d. See also supplementary material Fig. S1 and Movie 1.