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. 2014 Mar 15;141(6):1313–1323. doi: 10.1242/dev.104646

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Validation of methylation at novel gDMRs in gametes and effects of DNMT3L loss. (A) COBRA of methylation in wild-type (WT) and DNMT3L-deficient (3L) mouse oocytes. un, uncut; u, unmethylated; asterisk, limit digest band. (B) Clonal analysis, with methylation as a percentage of all sites indicated. Arrowheads indicate sites also analysed by COBRA. (C) As for A. Post-impl, genes known to be methylated only after implantation. (D) In situ hybridisation of testis tissue. Arrow indicates spermatogonial stem cells; arrowhead indicates spermatozoa. (E) Methylation of gDMRs as detected by pyrosequencing in sperm and oocytes. (F) RT-qPCR showing transcript levels in WT and 3L KO ovaries for the indicated genes. Error bars indicate s.e.m. ^**P<0.01, ^***P<0.001 by t-test.