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. 2014 Mar 15;141(6):1313–1323. doi: 10.1242/dev.104646

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Methylation and repression at novel gDMRs in adult mouse tissues. (A) COBRA on the adult mouse tissues and cell lines indicated at the top. Fibro, fibroblasts without (-) or with (+aza) 5-aza-2′-deoxycytidine. (B) Methylation analysis by pyroassay. (C) All analysed genes showed significant demethylation (P<0.05, ANOVA) by pyroassay following Aza treatment. (D) RT-PCR for the indicated genes in treated fibroblasts. Testis is a positive control (Pos ctrl), except for Adcy6 and Grin3b, where brain was used. IAP and Sycp3 are known to be repressed by methylation. Asterisked genes have low-density CGIs. Hprt is a loading control. (E) RT-qPCR of samples from D. UT, untreated. Error bars indicate s.e.m.