Fig. 4.

Conservation of methylation and repression in human. (A) Methylation levels as determined by pyrosequencing in hTERT-1604 normal human fibroblasts. (B) RT-PCR showing derepression by Aza treatment (lane +) is comparable between testis gDMR genes (left) and genes methylated postimplantation (right). Adult testis is a positive control; ACTB is for loading. (C) Significant differences in methylation were seen at all analysed genes in cells exposed to Aza or depleted of DNMT1 by siRNA (P<0.05, except for FKBP6 with Aza which was not significant). (D) RT-qPCR for the same samples. All upregulation >5-fold was significant at P<0.05. Error bars indicate s.e.m.