Figure 3.

Inhibition study of GLP-1 secretion using lactisole. To test the inhibitory effect of the lactisole, lactisole was treated to the NCI-H716 cells. The BF hexane fraction (HFBF) was treated with 100, 200, and 500 μg/mL each to the differentiated NCI-H716 cells. GLP-1 secretion was compared with the negative control and positive control. 1% DMSO used as negative control and 2 mM Quinine (Q) used as positive control. Statistical significance was determined by a one-way ANOVA and the values are means ± SEM; *P < 0.05 and ***P < 0.0001 versus 1% DMSO. ^# P < 0.05 and ^## P < 0.001 lactisole and HFBF treated group versus only HFBF treated group.