Table 1.

Sequences and predicted lengths of PCR amplification products of oligonucleotide primers used

Target	Primer	Oligonucleotide sequence (5'-3')Sequence	Product size	Reference
stx	VT1	ATTGAGCAAAATAATTTATAT GTG	523 bp	21
	VT2	TGATGATGGCAATTCAGTAT	520 bp
eae	SK1	CCCGAATTCCGCACAAGCATAAGC	863 bp	22
	SK2	CCCGGATCCGTCTCGCCAGTATTCG
eae-α1	EAE-FB	AAAACCGCGGAGATGACTTC	820 bp	7
	EAE-A	CACTCTTCGCATCTTGAGCT
eae-α2	IH2498aF	AGACCTTAGGTACATTAAGTAAGC	517 bp	7
	IH2498aR	TCCTGAGAAGAGGGTAATC
eae-β1	EA-B1-F	CGCCACTTAATGCCAGCG	811 bp	7
	EAE-B	CTTGATACACCTGATGACTGT
eae-β2	EA-B2-F	CCCGCCACTTAATCGCACGT	807 bp	7
	EAE-B	CTTGATACACCTGATGACTGT
eae-γ1	EAE-FB	AAAACCGCGGAGATGACTTC	804 bp	7
	EAE-C1	AGAACGCTGCTCACTAGATGTC
eae-γ2/θ	EAE-FB	AAAACCGCGGAGATGACTTC	808 bp	7
	EAE-C2	CTGATATTTTATCAGCTTCA
eae-δ/κ	EAE-FB	AAAACCGCGGAGATGACTTC	833 bp	7
	EAE-D	CTTGATACACCCGATGGTAAC
eae-ε	EAE-FB	AAAACCGCGGAGATGACTTC	722 bp	7
	LP5	AGCTCACTCGTAGATGACGGCAAGCG
eae-ζ	Z1	GGTAAGCCGTTATCTGCC	206 bp	7
	Z2	ATAGCAAGTGGGGTGAAG
eae-η	EAE-FB	AAAACCGCGGAGATGACTTC	712 bp	7
	LP8	TAGATGACGGTAAGCGAC
eae-ι	EAE-FB	AAAACCGCGGAGATGACTTC	807 bp	7
	LP7	TTTATCCTGCTCCGTTTGCT
eae-λ	68.4F	CGGTCAGCCTGTGAAGGGC	466 bp	7
	68.4R	ATAGATGCCTCTTCCGGTATT
eae-μ	FV373F	CAACGGTAAGTCTCAGACAC	443 bp	7
	FV373R	CATAATAAGCTTTTTGGCCTACC
eae-ν	IH1229aF	CACAGCTTACAATTGATAACA	311 bp	7
	IH1229aR	CTCACTATAAGTCATACGACT
eae-ξ	EAE-FB	AAAACCGCGGAGATGACTTC	468 bp	7
	B49R	ACCACCTTTAGCAGTCAATTTG
ehxA	HlyA1	GGTGCAGCAGAAAAAGTTGTAG	1551 bp	23
	HlyA2	TCTCGCCTGATAGTGTTTGGTA
astA	EAST1	CCA TCA ACA CAG TAT ATC CGA	111 bp	24
	EAST2	GGT CGC GAG TGA CGG CTT TGT
EAF	EAF1	CAG GGT AAA AGA AAG ATG ATA A	397 bp	25
	EAF25	TAT GGG GAC CAT GTA TTA TCA
bfpA	EP1	AAT GGT GCT TGC GCT TGC TGC	326 bp	26
	EP2	GCC GCT TTA TCC AAC CTG GTA