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. 2013 Mar;11(3):185–194.

Table I.

Data for the studied markers in the Insulin Receptor (INSR), and Adiponectin (ADIPOQ) genes

Gene/SNP*
(SNP ID)
rs number Location
(Base change)
Reverse primer Forward primer PCR program
(35 cycles)
PCR fragment size (bp) Restriction enzyme, Incubation temperature Alleles: RFLP fragments size (bp)
 INSR/ NsiI rs2059806 Exon 8 (A/G) 5′-CGGTCTTGTAAGGGTAACTG-3′ 93°C, 45 s,56°C NsiI, 37°C NsiI, 37°C Allele G: 324
Allele A: 239
+ 85
5′-GAATTCACATTCCCAAGACA-3′ 30 s, 72°C 45 s
INSR/PmlI (rs1799817) Exon 17 (T/C) 5′-CCAAGGATGCTGTGTAGATAAG-3′ 93°C, 45 s,56°C 317 PmlI, 37°C Allele T: 317
Allele C: 274
+ 43
5′-TCAGGAAAGCCAGCCCATGTC-3′ 30 s, 72°C 45 s
ADIPOQ/SmaI (rs2241766) Exon 2 (T/G) 5′-GAAGTAGACTCTGCTGAGATG G-3′ 93°C, 45 s,56°C 372 SmaI, 30°C Allele T: 372
Allele G: 216
+156
5′-TATCAGTGTAGGAGGTCTGTGATG-3′ 30 s, 72°C 45 s
ADIPOQ/BsmI (rs1501299) Intron 2 (C/A) 5′-GGCCTCTTTCATCACAGACC-3′ 93°C, 45 s,58°C 196 BsmI, 37°C Allele A: 196
Allele C: 146
+ 50
5′-AGATGCAGCAAAGCCAAAGT-3′ 30 s, 72°C 45 s

* Single nucleotide polymorphisms.