Table I.
Data for the studied markers in the Insulin Receptor (INSR), and Adiponectin (ADIPOQ) genes
|
Gene/SNP*
(SNP ID) |
rs number |
Location
(Base change) |
Reverse primer Forward primer |
PCR program
(35 cycles) |
PCR fragment size (bp) | Restriction enzyme, Incubation temperature | Alleles: RFLP fragments size (bp) |
|---|---|---|---|---|---|---|---|
| INSR/ NsiI | rs2059806 | Exon 8 (A/G) | 5′-CGGTCTTGTAAGGGTAACTG-3′ | 93°C, 45 s,56°C | NsiI, 37°C | NsiI, 37°C | Allele G: 324 Allele A: 239 + 85 |
| 5′-GAATTCACATTCCCAAGACA-3′ | 30 s, 72°C 45 s | ||||||
| INSR/PmlI | (rs1799817) | Exon 17 (T/C) | 5′-CCAAGGATGCTGTGTAGATAAG-3′ | 93°C, 45 s,56°C | 317 | PmlI, 37°C | Allele T: 317 Allele C: 274 + 43 |
| 5′-TCAGGAAAGCCAGCCCATGTC-3′ | 30 s, 72°C 45 s | ||||||
| ADIPOQ/SmaI | (rs2241766) | Exon 2 (T/G) | 5′-GAAGTAGACTCTGCTGAGATG G-3′ | 93°C, 45 s,56°C | 372 | SmaI, 30°C | Allele T: 372 Allele G: 216 +156 |
| 5′-TATCAGTGTAGGAGGTCTGTGATG-3′ | 30 s, 72°C 45 s | ||||||
| ADIPOQ/BsmI | (rs1501299) | Intron 2 (C/A) | 5′-GGCCTCTTTCATCACAGACC-3′ | 93°C, 45 s,58°C | 196 | BsmI, 37°C | Allele A: 196 Allele C: 146 + 50 |
| 5′-AGATGCAGCAAAGCCAAAGT-3′ | 30 s, 72°C 45 s |
* Single nucleotide polymorphisms.