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. 2012 Sep 14;59(1):18–26. doi: 10.1262/jrd.2012-068

Fig. 3.

Fig. 3.

Bull sperms with TUNEL assay by fluorescence microscopy. a: DAPI stained sperm samples with the objective#1. b: TUNEL assay with the objective#2. c: Overlap of two pictures; white arrow indicates DNA fragmented sperm cells (apoptotic) which stained as green whereas blue star shows DNA integrity of the sperm (non-apoptotic) containing blue color.