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. 2012 Oct 19;59(1):40–48. doi: 10.1262/jrd.2012-136

Fig. 3.

Fig. 3.

BDA injection into the unilateral ARC of castrated male goats. A: Photomicrograph showing BDA histochemistry in a section containing the injection site of a representative goat (#210). The injection site (a dotted circle) can be identified as densely packed BDA-positive products with a track of the injector. B–D: Schematic coronal view illustrations of the caudal portion of the goat ARC. Black dots represent the schematic distribution and relative density of KNDy neurons, but the number of dots does not necessarily correspond to the actual number of KNDy neurons. The BDA injection site of each goat is shown by the dotted line with the goat identification number. Panels B and C are separated by approximately 600 µm, while panels C and D are separated by approximately 300 µm. ARC, arcuate nucleus; DMH, dorsomedial nucleus; LHA, lateral hypothalamic area; VMH, ventromedial nucleus; fx, fornix; ME, median eminence; pt, pars tuberalis; 3V, third ventricle.