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. 2012 Oct 19;59(1):40–48. doi: 10.1262/jrd.2012-136

Fig. 5.

Fig. 5.

Co-localization of BDA and either NKB or kisspeptin in the ME and ARC. A–C (#210): Confocal microscopic images showing a fiber located in the ventral part of the ARC containing both BDA- (A) and NKB (B)-positive products. C: Merged image of A and B. D–F (#205): Confocal microscopic images showing fibers concomitantly containing BDA (D)- and kisspeptin (E)-positive products in the external layer of the ME (eME). F: Merged image of D and E. Pt indicates the pars tuberalis. G and H (#210): Merged fluorescence photomicrographic images of sections dual labeled for BDA (green) and kisspeptin (red). G: Apposition of BDA/kisspeptin fibers (arrowheads) to a kisspeptin-ir neuron in the ARC contralateral to the injection site. H: Apposition of BDA-positive (kisspeptin negative, open arrowheads) fibers to a kisspeptin-ir neuron in the ARC ipsilateral to the injection site. I: A photomicrograph showing NKB-ir fibers passing through the ventral part of the ARC under the third ventricle (3V) of a castrated male goat.