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. 2012 Nov 9;59(1):85–91. doi: 10.1262/jrd.2012-142

Table 1. Oligonucleotide primers for real-time PCR analyses.

Name (GenBank accession No.) Sequence Product length (bp)
ACTB F: 5'- cgacatccgtaaggacctgt -3'
(NM_001081838) R: 5'- gtggacaatgaggccagaat -3' 192
GZMB F: 5'- tctgacagctgctc actgct -3'
(NM_001081881) R: 5'- cagtcagcttggcctttctc -3' 188
IL-2 F: 5'- ccttgcaaacagtgcaccta -3'
(NM_001085433) R: 5'- gcatttcctccagaggtttg-3' 221
IL-4 F: 5'- caaaacgctgaacaacctca -3'
(NM_001082519) R: 5'- ttgaggttcctgtccagtcc -3' 198
IL-12 F: 5'- cacctggaccacctcagttt-3'
(NM_001082511) R: 5'- acggtgctgctcttgtcttt-3' 203
IL-15 F: 5'- gaggctggcattcatgtctt -3'
(AY682849) R: 5'- cgtttctggactcatgcaaa-3' 232
IFNG F: 5'- tcagagccaaatcgtctcct-3'
(EU000434) R: 5'- cgctggaccttcagatcatt-3' 186
TGFB1 F: 5'- agttaagcgtggagcagcat -3'
(AF175709) R: 5'-ctggaactgaacccgttgat -3' 244
CCR4 (LOC100056549) F: 5'- tagacaccaccgtggatgaa-3'
(XM_001490244) R: 5'- gaattcccaagcagaccaaa-3' 154
CCR5 F: 5'- cagaaaaccgacgtgagaca -3'
(NM_001091534) R: 5'- gggagggtgagaaggaaaag-3' 191

F, Forward; R, Reverse.