Table 1. Oligonucleotide primers for real-time PCR analyses.
Name (GenBank accession No.) | Sequence | Product length (bp) |
ACTB | F: 5'- cgacatccgtaaggacctgt -3' | |
(NM_001081838) | R: 5'- gtggacaatgaggccagaat -3' | 192 |
GZMB | F: 5'- tctgacagctgctc actgct -3' | |
(NM_001081881) | R: 5'- cagtcagcttggcctttctc -3' | 188 |
IL-2 | F: 5'- ccttgcaaacagtgcaccta -3' | |
(NM_001085433) | R: 5'- gcatttcctccagaggtttg-3' | 221 |
IL-4 | F: 5'- caaaacgctgaacaacctca -3' | |
(NM_001082519) | R: 5'- ttgaggttcctgtccagtcc -3' | 198 |
IL-12 | F: 5'- cacctggaccacctcagttt-3' | |
(NM_001082511) | R: 5'- acggtgctgctcttgtcttt-3' | 203 |
IL-15 | F: 5'- gaggctggcattcatgtctt -3' | |
(AY682849) | R: 5'- cgtttctggactcatgcaaa-3' | 232 |
IFNG | F: 5'- tcagagccaaatcgtctcct-3' | |
(EU000434) | R: 5'- cgctggaccttcagatcatt-3' | 186 |
TGFB1 | F: 5'- agttaagcgtggagcagcat -3' | |
(AF175709) | R: 5'-ctggaactgaacccgttgat -3' | 244 |
CCR4 (LOC100056549) | F: 5'- tagacaccaccgtggatgaa-3' | |
(XM_001490244) | R: 5'- gaattcccaagcagaccaaa-3' | 154 |
CCR5 | F: 5'- cagaaaaccgacgtgagaca -3' | |
(NM_001091534) | R: 5'- gggagggtgagaaggaaaag-3' | 191 |
F, Forward; R, Reverse.