Figure 5.

ATE1 and myosin co-immunoprecipitate in murine platelets. Myosin was immunoprecipitated from lysates of ATE1 knockout (KO) and control platelets. The immunoprecipitates were fractionated by polyacrylamide gel electrophoresis and immunoblotted with myosin or ATE1 antibodies. On the left, an anti-myosin immunoblot demonstrates that myosin was equally immunoprecipitated from control and ATE1 knockout platelets. The immunoblot on the right demonstrates that the anti-myosin immunoprecipitates also contain ATE1.