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. 2014 Mar 5;8:64. doi: 10.3389/fnbeh.2014.00064

Figure 1.

Figure 1

Viral and bead injection sites for studying mPFC inputs to BLA. (A) Confocal image of a representative brain slice of an animal injected in the mPFC with rAAV-ChR2(H134R)-eYFP (green). Scale bar: 500 μm. (B) Confocal image of a 35° tilted horizontal brain slice of the BLA with mPFC projections (green) corresponding to the injection site of (A). Scale bar: 250 μm. (C) Confocal image of a representative brain slice with retrobead injection site restricted to the PL region of the mPFC (red). Scale bar: 250 μm. (D) Image of an ex vivo recorded retrogradely labeled PN in the BA. Scale bar: 10 μm. (E–G) Overlay of mPFC viral injection sites (green) with the mouse brain atlas for animals categorized as having main injection sites in (E) mPFC (n = 13), (F) PL (n = 17) and (G) IL (n = 2). (H–J) Overlay with the mouse brain atlas for animals categorized as having the main retrobead injection site in (H) mPFC (n = 2), (I) PL (n = 13) and (J) IL (n = 2).