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. 2014 Mar 5;8:64. doi: 10.3389/fnbeh.2014.00064

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Copyright © 2014 Hübner, Bosch, Gall, Lüthi and Ehrlich.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Viral and bead injection sites for studying hippocampal inputs to BLA. (A) Stereoscopic picture of a representative brain slice of an animal injected in the ventral hippocampus (vHC) with rAAV-ChR2(H134R)-eYFP (green). (B) Overlay of vHC viral injection sites with the mouse brain atlas for all animals analyzed (n = 23). (C) Stereoscopic picture of a representative brain slice with retrobead injection site in the mPFC (red) of the same animal. (D–F) Overlay of main retrobead injection sites with the mouse brain atlas for all animals categorized as having the main injection site in (D) mPFC (n = 2), (E) PL (n = 5) and (F) IL (n = 8). (G) Confocal image of a coronal brain slice of the BLA with vHC projections (green) and retrogradely labeled principle neurons projecting to the mPFC (red) of the animal shown in (A) and (C). Scale bar: 250 μm. (H) Close-up of insert from (G) with vHC projections and retrobead-labeled neurons in the medial BA. Scale bar: 20 μm.