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. 2014 Mar 5;5:74. doi: 10.3389/fpls.2014.00074

FIGURE 1.

FIGURE 1

Immunolocalization of calreticulin (CRT) in longitudinal sections of C. glauca nodules embedded in Steedman’s wax (for the method, see Zdyb et al., 2011; for the antibody, see Baluška et al., 1999). Fluorescence micrographs (A,C) and differential interference contrast micrographs (B,D) are shown. The lignified walls of infected cells fluoresce in yellow under blue light. (A,B) No CRT labeling is found in walls (see arrow) between infected cells (ic). (C,D) CRT is labeled in walls connecting uninfected cells (uic) in a punctate pattern. Size bars denote 20 μm.