Table 1.
Binding Affinities of the Charged-to-Uncharged γ′ Peptides
| Peptide | Sequence | Kd (μM) (mean ± standard error of the mean) | P value (compared to wild-type)* |
|---|---|---|---|
| Wild-type | PEHPAETEpYDSLpYPEDDL | 0.440 ± 0.011 | – |
| #1 | PQHPAETEpYDSLpYPEDDL | 0.441 ± 0.016 | 1.00 |
| #2 | PEHPAQTEpYDSLpYPEDDL | 0.485 ± 0.011 | 0.46 |
| #3 | PEHPAETQpYDSLpYPEDDL | 0.541 ± 0.010 | 0.025 |
| #4 | PEHPAETE YDSLpYPEDDL | 0.583 ± 0.011 | 0.008 |
| #5 | PEHPAETEpYNSLpYPEDDL | 0.705 ± 0.011 | <0.001 |
| #6 | PEHPAETEpYDSL YPEDDL | 0.702 ± 0.021 | 0.018 |
| #7 | PEHPAETEpYDSLpYPQDDL | 0.582 ± 0.018 | 0.073 |
| #8 | PEHPAETEpYDSLpYPENDL | 0.608 ± 0.019 | 0.053 |
| #9 | PEHPAETEpYDSLpYPEDNL | 0.465 ± 0.010 | 1.00 |
| #10 | PEHPAETE YNSL YPEDDL | – | – |
| #11 | PQHPAQTQpYDSLpYPQNNL | – | – |
| #12 | PQHPAQTQpYDSLpYPEDDL | 1.968 ± 0.025 | <0.001 |
| #13 | PEHPAETEpYDSLpYPQNNL | 3.189 ± 0.034 | 0.002 |
BIAevaluation software was used to calculate affinity constants (Kd) based on a 1:1 Langmuir binding model. Standard error of the mean was calculated from triplicate determinations.
*
P values were adjusted for multiple comparisons using Bonferroni correction.
This potential individual and institutional conflict of interest has been reviewed and managed by OHSU.