Fig. 3.
Total Wwox ablation using EIIA-cre transgenic mice. Deletion of Wwoxfl/fl alleles was validated using a general deleter mouse strain carrying the Cre-recombinase gene under control of the adenoviral EIIA-promoter. A: Real-time PCR of mouse tissues isolated from control Wwoxfl/fl (WWOX) and Wwoxfl/fl –EIIA-cre (WwoxΔ/Δ) mice. Ubc levels was used for normalization. B: Western blot analysis of liver tissues using anti-WWOX antibody showing deletion of WWOX in WwoxΔ/Δ mice. GAPDH levels were used for normalization. C: Immunohistochemical staining of bone tissues using anti-WWOX antibody showing absent WWOX expression (brown) in WwoxΔ/Δ mice. D: A photograph showing growth retardation of WwoxΔ/Δ mice (right) as compared to Wwoxfl/fl control mice (left) at age 18 days.