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. 2014 Feb 8;14:30. doi: 10.1186/1471-2180-14-30

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Copyright © 2014 Du et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterization of the genes encoding hydroxylases or oxygenases. A, LC-MS analysis (extracted ion chromatograms of m/z [M + H]⁺ 969.5 corresponding to PLYA) of Streptomyces sp. MK498-98F14 wild type (WT) and mutants (ΔplyE, ΔplyP, ΔplyR, Δorf4, and ΔplyM). B, LC-MS analysis (extracted ion chromatograms of m/z [M + Na]⁺ 975.5 and 991.5 corresponding to PLYB and PLYA) of Streptomyces sp. MK498-98F14 wild type (WT) and the ΔplyM mutant. C, LC-MS analysis (extracted ion chromatograms of m/z [M + Na]⁺ 959.5 corresponding to the putative biosynthetic intermediate of PLYA lacking two hydroxyl groups) of Streptomyces sp. MK498-98F14 wild type (WT) and mutants (ΔplyE, ΔplyP, ΔplyR and ΔplyM). B was performed under the conditions: 35-95% B (linear gradient, 0–20 min), 100% B (21–25 min), 35% B (25-40 min) at the flow rate of 0.3 mL/min.