Table 1.
Deduced functions of ORFs in the biosynthetic gene cluster of PLYA
| Gene | Sizea | Accession no. | Proposed function | Homologous protein species | Identity/Similarity |
|---|---|---|---|---|---|
|
orf03399 |
384 |
YP_003099796 |
Nucleotidyl transferase |
Actinosynnema mirum DSM 43827 |
64/73 |
|
orf03396 |
309 |
YP_004903951 |
putative sugar kinase |
Kitasatospora setae KM-6054 |
50/62 |
|
orf1 |
422 |
YP_003099794 |
3-dehydroquinate synthase |
Actinosynnema mirum DSM 43827 |
56/69 |
|
orf2 |
128 |
EID72461 |
MarR family transcriptional regulator |
Rhodococcus imtechensis RKJ300 |
71/83 |
|
orf3 |
146 |
ZP_09957194 |
Hypothetical protein |
Streptomyces chartreusis NRRL 12338 |
75/84 |
|
orf4 |
566 |
CAJ61212 |
Putative polyketide oxygenase/hydroxylase |
Frankia alni ACN14a |
77/83 |
|
orf5 |
377 |
ZP_04706918 |
Alcohol dehydrogenase BadC |
Streptomyces roseosporus NRRL 11379 |
76/86 |
|
orf6 |
312 |
ZP_06582592 |
3-oxoacyl-[acyl-carrier-protein] synthase III |
Streptomyces roseosporus NRRL 15998 |
71/82 |
|
orf7 |
82 |
ZP_04706920 |
Hypothetical protein |
Streptomyces roseosporus NRRL 11379 |
59/75 |
|
orf8 |
82 |
ZP_04706921 |
Dihydrolipoamide succinyltransferase |
Streptomyces roseosporus NRRL 11379 |
65/81 |
|
orf9 |
326 |
ZP_06582595 |
2-oxoisovalerate dehydrogenase |
Streptomyces roseosporus NRRL 15998 |
75/87 |
|
orf10 |
303 |
ZP_04706923 |
Pyruvate dehydrogenase |
Streptomyces roseosporus NRRL 11379 |
74/84 |
|
plyA |
71 |
YP_640626 |
MbtH-like protein |
Mycobacterium sp. MCS |
80/87 |
|
plyB |
225 |
YP_712760 |
Putative regulator |
Frankia alni ACN14a |
76/84 |
|
plyC |
528 |
YP_712761 |
A |
Frankia alni ACN14a |
77/85 |
|
plyD |
77 |
YP_712762 |
PCP |
Frankia alni ACN14a |
85/94 |
|
plyE |
395 |
YP_712763 |
Putative hydroxylase |
Frankia alni ACN14a |
76/86 |
|
plyF |
2583 |
ABV56588 |
C-A-PCP-E-C-A-PCP |
Kutzneria sp. 744 |
56/68 |
|
plyG |
2809 |
ZP_05519638 |
C-A-PCP-E-C-A-PCP |
Streptomyces hygroscopicus ATCC 53653 |
73/82 |
|
plyH |
1662 |
BAH04161 |
C-A-M-PCP-TE |
Streptomyces triostinicus |
72/82 |
|
plyI |
247 |
YP_712767 |
TE |
Frankia alni ACN14a |
80/87 |
|
plyJ |
312 |
YP_003112824 |
Daunorubicin resistance ABC transporter |
Catenulispora acidiphila DSM 44928 |
78/90 |
|
plyK |
253 |
YP_712769 |
ABC transporter system |
Frankia alni ACN14a |
71/81 |
|
plyL |
1043 |
YP_003112826 |
Transcriptional regulator |
Catenulispora acidiphila DSM 44928 |
72/80 |
|
plyM |
412 |
AAT45271 |
Cytochrome P450 monooxygenase |
Streptomyces tubercidicus |
43/59 |
|
plyN |
450 |
ZP_04604097 |
Aminotransferase class I and II |
Micromonospora sp. ATCC 39149 |
58/70 |
|
plyO |
308 |
ZP_03862696 |
Polyketide fumonisin |
Kribbella flavida DSM 17836 |
48/64 |
|
plyP |
270 |
ZP_04292518 |
L-proline 3-hydroxylase type II |
Bacillus cereus R309803 |
32/51 |
|
plyQ |
88 |
YP_712776 |
PCP |
Frankia alni ACN14a |
76/89 |
|
plyR |
415 |
YP_712777 |
Cytochrome P450 monooxygenase |
Frankia alni ACN14a |
77/85 |
|
plyS |
245 |
AAT45287 |
TE |
Streptomyces tubercidicus |
70/81 |
|
plyT |
1031 |
YP_712779 |
KS-AT-ACP |
Frankia alni ACN14a |
71/80 |
|
plyU |
1872 |
YP_712780 |
KS-AT-DH-KR-ACP |
Frankia alni ACN14a |
69/79 |
|
plyV |
2199 |
YP_712781 |
KS-AT-DH-ER-KR-ACP |
Frankia alni ACN14a |
70/78 |
|
plyW |
1041 |
YP_712782 |
KS-AT-ACP |
Frankia alni ACN14a |
72/82 |
|
plyX |
1080 |
YP_712783 |
C-A-PCP |
Frankia alni ACN14a |
62/72 |
|
plyY |
253 |
ZP_04472110 |
TE |
Streptosporangium roseum DSM 43021 |
70/78 |
|
plyZ |
79 |
YP_001612061 |
LysR family transcriptional regulator |
Sorangium cellulosum |
65/77 |
|
orf11 |
287 |
YP_702564 |
Hypothetical protein |
Rhodococcus jostii RHA1 |
56/73 |
| orf14742 | 170 | YP_002777514 | Hypothetical protein ROP_03220 | Rhodococcus opacus B4 | 51/63 |
aNumbers are in amino acids.